KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). In this product video, see how the SpectraMax M5 quickly reads samples. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Multimode Reader Comparison Chart. Eliminate time-dependent substrate addition steps. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Average 98 stars, based on 1 article reviews. Multimode Readers Comparison Chart. 3: The cuvette chamber. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. 7 Figures from 1126 Publications Promega. Download PDF. The corrected absorbance is within 5% of the. The assay can accurately test the toxicology of compounds as shown in Figure 1. The. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. Cell viability는 세포막 Integrity 또는. GloMax Discover System. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. . Filterless readers provide consistent results with steady temperature regulation. SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. . **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. Unplug the power cable from the back of the instrument. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. More Product Information. Read modes. Description 1. . SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. SpectraMax i3x Multi-Mode Detection Platform. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Shake intensity. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The dual auto injector module. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. Learn more about its features, specifications, and applications in this user guide. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Failed to load PDF file. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. . Spectranet customer portalThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 304 signal level saturation. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. However, no piece of equipment is immune to breaking down. . Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. 312 gain calibration failed. Supplier: Molecular Devices. SpectraMax i3x Multi-Mode Detection Platform User Guide. . SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Prepare your iD series instrument for shipment. Time-. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. Instrument. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Example: [Drawer]. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. SpectraMax® Microplate Readers and SoftMax® Pro Software. *On the SpectraMax i3x reader only. ü; ü. i3x Multi-Mode Microplate Reader. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The user guide also covers the features and specifications of the SpectraMax MiniMax 300 Imaging Cytometer, an optional accessory that. Products : SoftMax Pro GxP Software, SoftMax Pro Software. A: percent GFP-positive cells calculated using the classification feature in the software. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Prepare your i3/i3x instrument for shipment. Find regional support. NORTH AMERICA. Controls containing all reaction. Image analysis provides accurate data. Danaher Inc spectramax i3x. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Thetypeof luciferase(Renilla. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). \r Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48 AM The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Results were plotted as ratio of green/red vs. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Amplitude : 1. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Bulk Kit. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. Italic and boldface type are used for emphasis. SpectraMax i3, i3x, and iD5 readers. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. . 4. 303 unable to cal dark current. As. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. to View Figures. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Also refer to this Multimode Reader Comparison Chart. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. 0´ 104 8. viii SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 340PC control panel are shown in boxed Helvetica type. Overview of Western Blot Cartridge. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Table 1. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. 0´ 104 4. 01 0. 2. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. HTRF IP standard curve. Shaking and incubations were performed using an on-deck orbital shaker. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Examples: “Press carefully to engage,” “ Do not press down. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Videos & Webinars. Find regional support. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Description 1. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Other technologies such as imaging. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. Molecular Devices Software. Find regional support. Specifically, we present neurite. Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. Wokingham, Berkshire RG41 5TS United Kingdom. Binding and Inhibition. 2. Cell counters for accurate and. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). 313 reference gain check fail. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Unparalleled performance on a personalized platform. Methods. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. SpectraMax iD3 and iD5 Register or Sign In. Both readers feature an 8-channel read head for fast reads and. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 58% for the detection module and 2. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. Replicate values were averaged and imported into Design-Expert 8. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 10. Description 1. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. Bioz Stars score: 98/100, based on 1 PubMed citations. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Background Parallax,Columns 2. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. Find our technical support information along with contact details. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Supported Windows Operating System: See release notes. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. 22 μ m fi ltration. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Molecular Devices ScanLater Western Blot Detection Platform. infoboxeu@moldev. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. 7 Figures from 1126 Publications. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Example: [Setup]. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. gen) on a Spectramax i3X luminometer. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. FlexStation® 3 Multi-Mode Microplate Reader. Spectranet customer portal. 2 Parallel Line Analysis Conc log [ng/ML] RLU. SoftMax Pro. 0´ 104 6. ü; SpectraMax® Mini reader support. each new assay. Find our technical support information along with contact details. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 1321N1, a human astrocytoma cell line. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. Our absorbance plate readers feature our PathCheck Sensor. SpectraMax® L reader support; ü. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0 mm hex key. Simplified workflow: Add cellular imaging assays in a small footprint. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. We would like to show you a description here but the site won’t allow us. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. *On the SpectraMax i3x reader only. Monday to Friday 7am - 5pm Pacific Time. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Microplate readers, multi-mode, SpectraMax® i3x. 100 fmol 1536-well (8 µL) 0. Controls containing all reaction. Molecular Devices. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 5 decades, with sensitive detection of cells. Prime function 20 amol. Molecular Devices are known for both the reliability and the wide uses of their products. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors; It allows you to explore cellular pathways and protein activation and expression in one system;In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The chamber. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. . Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. Both Microplate Optimization and Read Height Adjustment were. . AlphaScreen. Viewing this answer requires an authorized account. Curcumin binding assay. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Pick the best speed, sensitivity, and price for your needs. Wait 20 seconds. Note: Additional readers with similar performance are listed. Catalog excerpts. Microplate reader assays requiring infrared (IR) or red-shifted. Bioz Stars score: 98/100, based on 1 PubMed citations. 5577 Web: Email: info@moldev. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. 0´ 104 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Summary of Contents for Molecular Devices SpectraMax M2. ZERO BIAS - scores, article reviews, protocol conditions and more. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. The calculated LOQ values were 0. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Description 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. SpectraMax ® iD3 and iD5 Multi-Mode . SoftMax Pro User’s Manual v Contents Figures. Price from $9. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Sensitive TRF detection of two simple, mix-and-measure assay workflows. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. ; The top 3 importers of Spectramax are India with 365. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. SpectraMax Microplate Readers. 99 to $1999. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 313 reference gain check fail. Winnersh Triangle. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. StakMax Handling Stacker Base Unit. For wavelengths in the UV range above 220 nm,. 5. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Description 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. . Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. D 1 1. Order Now. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Multimode Reader Comparison Chart. SPECTRAMAX i3X, INJECTOR MODULE CARTRIDGE model number 0200-7029. 1 Microplate Data Acquisition & Analysis Software for Windows. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. . SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. Use the 3. It allows you to explore cellular pathways and protein activation and expression in one system. Average 98 stars, based on 1 article reviews. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. . Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,(foc) spectramax i3x alphascreen 384 std detection cartridge - p/no:0200-7017 (cartridge for demonstration purpose) united kingdom: chennai air cargo: unt: 1: 1,254,478: 1,254,478: aug 04 2016: 90273090: spectramax i3x multi-mode microplate reader; iqoq for spectramax i3x smp7 gxp license(for r&d lab use only) united states: bombay air cargo. Microplate Readers SpectraMax. i3x single use: $35: $52. Cet appareil ISM est confomre à la norme NMB-001 du Canada. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Settings not required by a reader are indicated by ‘---’. . Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). Explore a wealth of applications with the future-ready SpectraMax i3x. Table 1. 5 Figures from 202 Publications Thermo Fisher Scientific. User-upgradeable detection modules allow you to expand the capabilities of your microp. Align the two slots on the bottle holder with the two pegs on the rail. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Danaher Inc spectramax i3x. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Description 1. ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. Introduction. The calculated LOQ values were 0. . SpectraMax Quant dsDNA Assay Kits. Molecular Interactions. Published Dec 16, 2020 | Updated Oct 10, 2023. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. china@moldev. +1 800-635-5577.